Surface Areas and Recommended Medium Volumes for Corning® Cell Culture Vessels
Introduction
This guide gives the recommended medium volumes, approximate growth surface areas and average cell yields for Corning disposable cell culture vessels.
Approximate growth surface areas are based on calculations made from engineering drawings. These calculations do not take into consideration minor variations that can occur in products during molding or the ability of many cell lines to grow up the sides of the vessels which can considerably increase the available surface area. For critical work, we suggest that you fix and stain cultures and then carefully calculate the actual growth surface area.
In general, at least 1 x 105 cells/cm2 can be produced when growing cells as attached monolayers in culture. The average cell yields used here are based on this number. Actual cell yields can easily be several times higher or lower than this depending on the cell line and culture conditions.
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