{"id":1837,"date":"2013-07-01T16:06:27","date_gmt":"2013-07-01T15:06:27","guid":{"rendered":"http:\/\/www.nutrigenomiclab.com\/lab\/?p=1837"},"modified":"2013-07-01T16:07:52","modified_gmt":"2013-07-01T15:07:52","slug":"surface-areas-and-medium-volumes-for-cell-culture-vessels","status":"publish","type":"post","link":"https:\/\/www.nutrigenomiclab.com\/lab\/?p=1837","title":{"rendered":"Surface Areas and Medium Volumes for Cell Culture Vessels"},"content":{"rendered":"<p><strong>Surface Areas and Recommended Medium\u00a0Volumes for Corning\u00ae Cell Culture Vessels<\/strong><\/p>\n<p>Introduction<br \/>\nThis guide gives the recommended medium volumes, approximate growth surface areas and\u00a0average cell yields for Corning disposable cell culture vessels.<br \/>\nApproximate growth surface areas are based on calculations made from engineering drawings.\u00a0These calculations do not take into consideration minor variations that can occur in products\u00a0during molding or the ability of many cell lines to grow up the sides of the vessels which\u00a0can considerably increase the available surface area. For critical work, we suggest that you\u00a0fix and stain cultures and then carefully calculate the actual growth surface area.<br \/>\nIn general, at least 1 x 105 cells\/cm2 can be produced when growing cells as attached monolayers\u00a0in culture. The average cell yields used here are based on this number. Actual cell\u00a0yields can easily be several times higher or lower than this depending on the cell line and\u00a0culture conditions.<\/p>\n<p><a href=\"http:\/\/catalog2.corning.com\/Lifesciences\/media\/pdf\/cc_surface_areas.pdf\" target=\"_blank\">Link to the PDF<\/a><\/p>\n<p style=\"text-align: center;\"><a href=\"http:\/\/www.nutrigenomiclab.com\/lab\/wp-content\/uploads\/2013\/07\/platespecs.jpg\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter  wp-image-1838\" alt=\"platespecs\" src=\"http:\/\/www.nutrigenomiclab.com\/lab\/wp-content\/uploads\/2013\/07\/platespecs.jpg\" width=\"657\" height=\"406\" srcset=\"https:\/\/www.nutrigenomiclab.com\/lab\/wp-content\/uploads\/2013\/07\/platespecs.jpg 821w, https:\/\/www.nutrigenomiclab.com\/lab\/wp-content\/uploads\/2013\/07\/platespecs-300x185.jpg 300w\" sizes=\"(max-width: 657px) 100vw, 657px\" \/><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Surface Areas and Recommended Medium\u00a0Volumes for Corning\u00ae Cell Culture Vessels Introduction This guide gives the recommended medium volumes, approximate growth surface areas and\u00a0average cell yields for Corning disposable cell culture vessels. Approximate growth surface areas are based on calculations made from engineering drawings.\u00a0These calculations do not take into consideration minor variations that can occur in [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"ngg_post_thumbnail":0,"_links_to":"","_links_to_target":""},"categories":[1,8],"tags":[30,28,29],"_links":{"self":[{"href":"https:\/\/www.nutrigenomiclab.com\/lab\/index.php?rest_route=\/wp\/v2\/posts\/1837"}],"collection":[{"href":"https:\/\/www.nutrigenomiclab.com\/lab\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.nutrigenomiclab.com\/lab\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.nutrigenomiclab.com\/lab\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.nutrigenomiclab.com\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1837"}],"version-history":[{"count":3,"href":"https:\/\/www.nutrigenomiclab.com\/lab\/index.php?rest_route=\/wp\/v2\/posts\/1837\/revisions"}],"predecessor-version":[{"id":1840,"href":"https:\/\/www.nutrigenomiclab.com\/lab\/index.php?rest_route=\/wp\/v2\/posts\/1837\/revisions\/1840"}],"wp:attachment":[{"href":"https:\/\/www.nutrigenomiclab.com\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1837"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.nutrigenomiclab.com\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1837"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.nutrigenomiclab.com\/lab\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1837"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}